Data filtering

Filtering tab in the GUI

After resolving levels and fitting their lifetimes, the resulting statistics can be inspected and the data filtered in the Filtering tab. The available parameters are the number of photons in the level, the level intensity, the fitted (average) lifetime of the level, the Durbin-Watson parameter, the Chi-squared value and the fitted IRF shift. For example, clicking on “Intensity Distribution” and then on “Plot Distribution” shows the following:

Filtering tab in the GUI showing intensity distribution

Different values can also be viewed using a scatter plot by selecting the relevant features under “Two Feature Investigation” and clicking “Plot”. For example, an intensity-lifetime plot looks like this:

Filtering tab in the GUI showing scatter plot

The number of histogram bins is set automatically but can also be set manually under “#Bins” by first unselecting “Auto”. If “Only Drift” is checked, only intensities below the regression line will be normalised, else all intensities are normalised. Selecting “All” applies the normalisation to all levels and groups instead of only the currently displayed data. This data can also be filtered, for example based on minimum and maximum lifetime values:

Filtering tab in the GUI showing scatter plot

For an intensity-lifetime plot, the data can also be fit with a linear regression:

Filtering tab in the GUI showing scatter plot and linear fit

Finally, the intensities can be normalized to the fitted lifetime. If this is only done to correct for drift, “Only Drift” can be left selected before clicking “Apply Norm.”. This moves the points below the fit line onto the line:

Filtering tab in the GUI showing scatter plot

If “Only Drift” is unchecked, all points will be moved onto the line during normalization:

Filtering tab in the GUI showing normalized scatter plot